3. Ordering information. 4. Functional diagram. HEFB. Quad 2-input AND gate. Rev. 8 — 15 December Product data sheet. Table 1. Ordering. Details, datasheet, quote on part number: ZL Application Notes) Ordering Information ZL/DCE ZL/DCF (tubes) 8 pin SOIC (tape and reel) 8. Cat. No. / Cat. No. / System (w/micro CA). Cat. No. Cat. No. / Cat. No. / Removable Drum Only.
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Isotype controls should be concentration matched and run alongside the primary antibody samples. The optimal lysate concentration will depend on the expression datashset of the protein of interest. Pre-clear enough lysate for test samples and isotype controls.
Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818
Separate the beads from the lysate using a magnetic separation rack, transfer the pre-cleared lysate to a clean tube, and discard the magnetic bead pellet. Adjust pH to 8. Repeat washing step once more. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. Detection of Proteins Directions for Use: Cells should be grown, treated, fixed ddatasheet stained directly in multi-well plates, chamber slides or on coverslips.
Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube.
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Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Incubate with rotation for 20 min at room temperature.
It should be noted that for the best possible results a fresh blot is always recommended. Analyze cells in DNA staining solution on flow cytometer. Blotting Membrane and Paper: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different datasheey membranes, adjust volumes accordingly.
Biotinylated Protein Ladder Detection Pack: Datasehet magnetic beads just prior to use: Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
Fix for 15 min at room temperature. Find answers on our FAQs page. Mix well then add 0. Incubate for 1 hr at room temperature.
CST – Phospho-STING (Ser) (D8K6H) Rabbit mAb
Pre-wash magnetic beads see Cell Lysate Pre-Clearing section, steps 1 and 2. Wash by centrifugation with excess 1X PBS. Place the tube in a magnetic separation rack for seconds. If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
Wash three times for 5 min each with 15 ml of TBST. Mizuno E et al. Vortex, then microcentrifuge for 30 sec. Primary Antibody Dilution Buffer: Block specimen in Blocking Buffer for 60 min. Incubate for 30 min at room temperature.
To Purchase S View sizes.
Do not aliquot the antibody.